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Potential experimental approaches for functional characterization of uPE1 proteins. This can be divided into two workflows, PPI-based function prediction, and Multi-Omics Knowledge-based function prediction. The prediction results can further be tested by using Phenotypic Cell-based Screens that can utilize biochemical or immunologic assays for verification of the prediction results. These experiments can be used in a flexible manner as appropriate to verify and validate the function(s) of target dark proteins. Functionally validated dark uPE1 proteins will be promoted to PE1 and curated by neXtProt. (from Paik et al., J. Proteome Res. 2018, 17, 4042−4050)